As with any fluorochrome used in a flow cytometric assay, the success of FP detection depends on the level of FP expression within the cells, the excitation wavelength used and the light filters placed in front of the fluorescence detectors. Nos. Such wear includes scratches, scrapes, and general dirtiness. Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP. BD Accuri C6 Flow Cytometer Instrument Manual 7820018 Rev-4 1 1 INTRODUCTION The BD Accuri™ C6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. BD offers a selction of inferior vena cava filter products. 661085). A unique low-pressure pumping system drives the fluidics. • Replace all fluid filters … The BD Accuri C6 is a compact flow cytometer that uses a low-pressure pumping system to drive the fluidics allowing for the derivation of sample volume and calculation of absolute counts or sample concentration per microliter. C6 Flow Cytometer 1-year Maintenance Kit . Clinical Research and the Rockefeller University Hospital, Chemers Neustein Summer Undergraduate Research Fellowship Program, Experience Science, the Arts, and Culture. 29 0 obj <> endobj The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. A sheath-focused core enables event rates of up to 10,000 events per second and a sample concentration of over 5 x 106 cells per mL. The default configuration of the BD Accuri ® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. All reagents and kits are compatible with both the BD Accuri C6 Plus and BD Accuri C6 flow cytometer systems. Always protect the Sample Introduction Probe (SIP) with a tube of HPLC reagent grade filtered, deionized H 2 O (0.22 µm filter), or equivalent, when the cytometer is not running samples. %PDF-1.6 %���� Rockefeller University You are viewing an Accuri C6 Flow Cytometer. Designed from the ground up to be compact and easy to use, the BD Accuri C6 flow cytometer fits in any lab and is readily accessible The BD Accuri ® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. 52 0 obj <>/Filter/FlateDecode/ID[<6D17E613E92D6948AEF4F9A1572DFD51>]/Index[29 41]/Info 28 0 R/Length 106/Prev 60895/Root 30 0 R/Size 70/Type/XRef/W[1 2 1]>>stream All rights reserved. the Accuri C6 Flow Cytometer System. The ideal cell concentration h�b``�```�����`��π ���@q��L�n\g���`��h 'c�. Light, rugged, and small enough to fit on a benchtop, it connects to standard electrical circuitry and can easily be transported to a water treatment facility or into the field. Data is digitally collected over a 7-decade dynamic range (16 million channels of digital data), making all data available to users as needed. In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. Table of Contents. Select “Tools” from the menu bar. Analytical Flow Cytometry - Accuri C6 Configuration Please note that the Accuri C6 is for use by trained personnel only! The non-pressurized system of BD Accuri C6 supports any brand of sample tubes – 12 x 75 mm or smaller (including microcentrifuge tubes), made of polypropylene or polystyrene. BD LSRFortessa 2. BD Accuri™ C6 Flow Cytometer Features: Equipped with a blue and a red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. Contact, Flow Cytometry Resource Center (FCRC) Copyright 2004—2020 The Rockefeller University. ... optical filters optimized for the detection of FITC, PE, PerCP, and APC. Which fluorochromes are compatible with the cytometers? Filter and Fluorochrome List for the Accuri® C6 Flow Cytometer® System ©January, 2010 Standard Filters Optional Filters (other) Fluorescent Protein Filters (for simultaneous GFP/YFP detection) FL1 530 +/- 15nm FL1 510 +/- 7.5 nm 510 +/- 7.5 nm FL2 585 +/- 20 nm FL2 565 +/- 10 nm 540 +/- 15nm FL3 670nm LP FL3 610 +/- 10 nm 670nm LP To view hidden file extensions which distinguish BD Accuri C6 Software files (.c6) from BD Accuri C6 Software template files (.c6t): 1. A compact optical design, fixed 2. 69 0 obj <>stream This item has been powered on, but no further testing. BD LSRFortessa 1. The Accuri C6 Flow Cytometer does not produce aerosols during normal operation. Easy to use - fixed alignment and pre-optimized detector settings. 0 filters. The latest science discoveries delivered monthly to your inbox. The BD Accuri® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. Waste and sheath fluid bottles were checked during this time, and emptied or refilled as required. You can also set custom speeds/core sizes up to a 40 micron core, that might help the clogging issue with your 30 micron cells. A unique, non-pressurized, peristaltic pump system drives the fluidics. Researchers at the Swiss Federal Institute of Aquatic Science and Technology (Eawag, Switzerland) have developed a standard flow cytometric staining protocol and a corresponding BD AccuriTM C6 software analysis template to discriminate bacteria from debris in drinking water samples. Note: This is not a full list of all fluorochromes that are compatible with the flow cytometers. The standard optical filters in the BD Accuri C6 Plus are optimized to detect common fluorochromes such as FITC/BB515, PE, PerCP, and APC. In addition, the system derives sample volume and can calculate absolute counts or sample concentration per microliter. Meet the scientific leaders who are changing medicine, Peek inside our 70 biomedical laboratories, Learn more about our flexible, supportive academic programs, Learn about the breakthroughs happening every day, Hear from the world’s leading speakers and thinkers, We’ve spent 119 years perfecting the bioscience institute. BD Accuri® C6 CFlow SAMPLER supports the high throughput and fully automated acquisition from 48- and 96-well plates and deep-well plates. The running costs are very low as it doesn¹t use any proprietary sheath fluid or reagents. - Find MSDS or SDS, a COA, data sheets and more information. endstream endobj startxref A compact optical design, fixed alignment, and pre- No. The BD Accuri® C6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. Overall very good cosmetic condition. Additional laser configurations (2-blue 2-red, 4-blue) are available with the Selectable Lasers Module (Cat. Since yeasts are relatively large (I use an Accuri C6 to count bacteria so yeast are large to me) I would go with the faster/larger core size. The Accuri C6 cytometer in my lab has some problems, and we need some help. Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube. Higher concentrations might result in inaccurate counting due to system saturation, so dilute samples if necessary. ... filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. A compact optical design. For any system, we recommend: • Check all fluidic tanks for contamination and replace all cubitainers as applicable. Phase III+: The University is open for expanded research operations; only authorized personnel will be admitted on campus. The default configuration of the BD Accuri® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. When we turn on, the system does not collect liquid as normally, in fact is completely silent (no peristaltic pump working). Came with note that it needs repair. Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). -Ģ �r^�F)�?2��7�% �K��x4JR���E�Y��Wl������3� �;p�y[�AD= r This Technical Note To increase signal resolution, or to separate fluorochromes with overlapping signals, a choice of optional, user-interchangeable filters is available. 653146, 653148, and 653147 Sample Preparation Volume measurements on the BD Accuri C6 are most accurate with cell concentrations between 1,000 and 5000000 cells/mL. Millex Sterile syringe filter units are available in a larger 33mm housing with either MCE, PVDF or PES membranes. h�bbd``b`:$� -�8�`�$XJA�H�H0'��U �.+ ... considering that we still have to buy the replacement kit with filters, inline filter, and rubber tubbings. hެ�mS�8ǿ�^��[���L��@H�]�LC�w���$J�c����O�R�B ��:aGZ�V��6h���eZ3.ӆ !�N��Ӗ).�Θ����Y�2��V2#W�%㙱�(&���h&2�OäV 3��b#�(!5{���W�֕78���L�/��� Gը�_���~=ZU0�-a��aPW�Cw�����W8v4 �•���r���֍qlj�Į��!��e���N�f��p�c��ޟ-[ޟ�0��͵��I�~F�������ѭ3��w0�C�z�����?~������S�d��r��>U�j���~1���U#�^�&L�˥ӥM�����R)拷���. BD Accuri™ C6 software was started. %%EOF 1230 York Avenue, Box 344 BD Accuri C6 Plus fluorochrome support All fluorochromes in the table can be detected by the BD Accuri C6 Plus equipped with standard filters. Available with the Selectable Lasers Module ( Cat both the BD Accuri C6 Configuration Please note that Accuri... Kit with filters, inline filter, and BD Accuri C6 flow Cytometer ( Figure 2 ) performance. 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Fcr staff are here fluidic tanks for contamination and replace all cubitainers as applicable checked during this time and! Standardized fluorescence performance so that users do not need to adjust detector.... Or refilled as required 96-well plates and deep-well plates Please note that the Accuri C6 flow in... Scratches, scrapes, and APC optimized for the detection of FITC, PE, PerCP, and general.... The Rockefeller University Hospital, Chemers Neustein Summer Undergraduate Research Fellowship Program, Experience science, the Arts, Culture!, scrapes, and rubber tubbings that users do not need to detector. With Selectable Lasers Module ( Cat Cytometer does not produce aerosols during normal operation allowed to warm for! Cytometry - Accuri C6 … filters in use are identical to FACS Calibur further testing full of... Very low as it doesn¹t use any proprietary sheath fluid bottles were checked during this time, and Accuri. 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